López-Froilán, Rebeca; Hernández-Ledesma, Blanca; Cámara, Montaña; Pérez-Rodríguez, María Luisa.
Food Analytical Methods 11, 2897-2906.
https://doi.org/10.1007/s12161-018-1259-1
Resumen
Definition of a standard method for measuring antioxidant capacity is still a necessity for both researchers and food industry. This study is aimed to evaluate if Folin-Ciocalteu (F-C) method could be reconsidered as suitable for antioxidant potency comparison purposes in food matrices. F-C and four other common tests for measuring antioxidant capacity (ORAC, TEAC, FRAP, CUPRAC) were applied to mixed fruit-based beverages. An overall antioxidant potency composite index (API
composite) was calculated by assigning each test equal weight, in order to establish the ranking of antioxidant capacity in the analyzed samples. In addition, the study provides an evaluation of the compounds related to the antioxidant potential of these beverages (total phenolics, anthocyanins, and ascorbic acid) through principal component analysis (PCA).
Total phenolics were the principal component influencing the antioxidant capacity of these samples. This is highlighted with the high correlation found between FBBB and all the antioxidants assays performed (P<0.05). API composite and F-C assay were consistent in classifying the antioxidant capacity of these beverages in the following order: Red fruit formulated juices > orange fruit formulated juices > mixed fruit nectars. As a result, the use of F-C for antioxidant potency comparison of fruit-based beverages is encouraged.
